Determination of Resveratrol in Longlianlidan Mixture by HPLC

OBJECTIVE To establish an HPLC method for determination of resveratrol in Longlianlidan mixture. METHODS The determination of resveratrol in Longlianlidan mixture by HPLC was performed with HYPERSIL ODS C₁₈ (250 mm×4.6 mm, 10 µm) and the mobile phase of acetonitrile-water(25∶75) at a flow rate of 1.0 mL·min^–1, and the detection wavelength was conducted at 305 nm and at room temperature. RESULTS The calibration curve was linear within the range of 8.2-164 µg·mL^–1 and the average recovery of resveratrol was 84.56%, 88.31%, 90.67%, respectively. CONCLUSION The HPLC method is precise, quick and suitable for determination of resveratrol in Longlianlidan mixtrue.