Determination of Arctiin in Lingqiaojiedu pills by HPLC

OBJECTIVE To establish a HPLC method to determine arctiin in Lingqiaojiedu pills. METHODS Agilent EclipsexDB-C₈ (4.6 mm×150 mm, 5 μm) with temperature of 30 ℃. The mobile phase was CH₃OH: H₂O (48∶52), with flow rate of 0.8 mL·min^-1 and the detection wavelength was 280 nm. RESULTS This method had good resolution and good linear relationship between 0.017 0-0.085 1 g·L^-1. The average recovery of arctiin was 96.6% and RSD was 4.18%. CONCLUSION This method was simple and quick and can be used as the method for quantity control of Lingqiaojiedu pills.