Influence of Zuogui Pill on Sex Hormone in Peripheral Blood Serum of Immune Premature Ovarian Failure Rats

OBJECTIVE To explore the effect of Zuogui pill (ZGP) , a Chinese compound recipe for tonifying Shen, on AZpAb，FSH, E₂ in peripheral blood serum of immune premature ovarian failure (POF) rats and to further reveal the mechanism in regulating ovarian function in rats. METHODS BALB/C grade SPF female mice model of POF was established by multiple sites subcutaneous injection of zona pellucida 3 of mice, and treated with different dosage ZGP of low, middle and high one for 4 weeks, with prednisone and diethylstilbestrol as positive control. Detected AZpAb，FSH, E₂ in peripheral blood serum by ELISA . RESULTS There was significantly different within groups FSH and AZpAb, and the FSH was significantly different in diaethylstilbestrol group, the E2 value was significantly different in ZGP of low and high dosage group, and it markedly different in AZpAb value in ZGP of low and high dosage and diaethylstilbestrol groups. CONCLUSION Zuogui pill may improve the immuno-inflammation damage in ovaries of POF rat by increasing the concentration of E2 value in peripheral blood and inhibiting AZpAb production.