The Effect and Mechanism of Luteolin on the Activity of Aldose Reductase from Cardiac Muscle of Rats

OBJECTIVE To investigate the intervention effect of luteolin on the activity of aldose reductase (AR) in spontaneously hypertensive rat(SHR), and to provide experimental evidence for study on the effect and mechanism of AR on attenuating hypertension-induced cardiovascular remodeling. METHODS Ultraviolet spectrophotometry was performed to determine the activity of AR from cardiac muscle of SHR. Immumofluorescence method were performed to determine the content of blood plasm active mass of AngⅡ. RESULTS The activities of AR (0.054 2±0.001 8) and AngⅡ(110.48±3.79) from cardiac muscle of SHR that had been induced by intragastric administration of luteolin were significantly lower than by intragastric administration of normal saline, and were higher than normal group(P<0.05). The changed tendency of AR activity were correlated with content of AngⅡ(r=0.968 6, P=0.04). CONCLUSION Luteolin can inhibit the activity of AR. The mechanism may be related to decreasing content of AngⅡ.