The Optimization of Extraction Method of Polysaccharides from Carapax Trionycis by Orthognal Test

OBJECTIVE To study the optimum extration process of polysaccharides from Carapax Trionycis. A spectrophotometry method for content determination of polysaccharides from Carapax Trionycis was established with Phenolsulfuric acid coloration. METHODS Polysaccharides was extracted by NaOH solution and alcohol precipitation from Carapax Trionycis. The contents of po1ysaccharides from Carapax Trionycis were determined by spectrophotometry at 490 nm．RESULTS The results of orthogonal test in treatment of alkali extraction showed the optimum conditions were as the follows: NaOH concentration was 8%, treatment temperature was 60 ℃, extraction time was 8 hours, the ratio of the material and the solution was 1∶25. The average recovery rate was 100.23% and RSD was 1.237%(n=9)．CONCLUSION The method is proved to be simple, accurate and reliable, and can be used to extract and determine the polysaccharides from Carapax Trionycis．