Study on Fingerprint Spectrum of Rhizoma Alismatis Extract by HPCE

OBJECTIVE To establish the fingerprint spectrum of Rhizoma Alismatis extract by HPCE．METHODS Using Capillary Zone Electrophoresis (CZE) patterns, injection pressure: 0.5Psi×10 s, separation voltage 30kV, column temperature 25 ℃, DAD detection wavelength 202nm. Running buffer 0.1 mol·L^-1 borax-0.1 mol·L^-1 SDS-methanol(4∶6∶2), and analysis the the similar by similar evaluated software. RESULTS All of the similarity are above 0.9, so the fingerprint integer feature of the 10 batches Rhizoma Alismatis extract are coincident essentially. CONCLUSION The established fingerprint spectrum is stable and credible, and could be used as characteristic fingerprint spectrum for Rhizoma Alismatis extract. And it will be a new reference for qualitation identification and quality evaluation of Rhizoma Alismatis extract.