Sephadex Chromatographic System for Determination of Polymer in Piperacillin Sodium/Tazobactam Sodium for Injection

OBJECTIVE To develop a method for determination of polymer in piperacillin sodium/tazobactam sodium for injection. METHODS Sephadex G-10 column was used with the mobile phase A of 0.01 mol·L^-1 phosphate buffer (pH 7.0) and mobile phase B of water. The flow rate was 1.0 mL·min^-1 and the wavelength was 254 nm. RESULTS The linear range of polmer in piperacillin sodium/tazobactam sodium for injection was 40.10-180.45 μg·mL^-1(r=0.999 6). CONCLUSION The method was convenient and accurate, and can meet the requirements of drug quality control.