Synthesis and Evaluation of Molecularly Imprinting Polymer Using Roxithromycin as Template

OBJECTIVE To synthesize molecularly imprinting polymer(MIP) for roxithromycin by using molecular imprinting technique(MIT), and study the characterisation of it. METHODS A MIP with special molecule recognition properties of roxithromycin was prepared by thermal polymerization in which roxithromycin as template molecule, α-methacrylic acid as functional monomer and ethylene glycol dimethacrylate as the crosslinker. RESULTS Equilibrium binding experiment was used to investigate the adsorption dynamics, the binding ability to template molecule and the substrate selectivity. The Scatchard analysis was used to study that the MIP's binding characteristic to template molecule. By the analysis of Scatchard plot, the apparent maximum number (Q_max) and the equilib-rium dissociation constant (Kd) were found to be 90.3 mg·g^-1 and 1.35 mg·mL^-1, respectively. CONCLUSION The MIP for roxithromycin we synthsized produces a cavity with molecular recognition sites that can bind selectivity to the template, that is to say that it has imprinting effectiveness.