Determination of Berberine Hydrochloride in Mixture for Burn and Scald by HPLC

OBJECTIVE To determine the content of berberine hydrochloride in mixture for burn and scald. METHODS The content of berberine hydrochloride in mixture for burn and scald was determined by HPLC with Inertsil C_8-3 (4.6 mm× 250 mm, 5 μm) column. The mobile phase was 0.05 mol·L^-1 sodium dihydrogen phosphate solution-acetonitrile-phosphoric (70∶30∶0.08) with a flow rate of 1 mL·min^-1, the columm temperature was set at 35 ℃ and the wavelength of the detector was set at 260 nm. RESULTS The linear range was 5.28-52.8 mg·L^-1 and the correlation cofficient was 0.999 4. The average recovery and the RSD were 100.30% and 1.24%(n=6). CONCLUSION The method is simple, accurate and repeatable. It is proved to be suitable for the quality control of mixture for burn and scald.