Determination of Trahydorpalmatine in Xuedan Weichang Pill by HPLC

OBJECTIVE To establish an HPLC method for determination of contents of trahydorpalmatine in Xuedan Weichang pill. METHODS To utilize RP-HPLC, the Hypersil ODS2 column (250 mm×4.6 mm, 5 μm) was used with the mobile phase of acetonitrile-0.1% phosphate acid (adjustd to pH 6.0 by triethylamine) (60∶40). The flow rate was 1.0 mL·min^-1. The detection wavelength was 225 nm. The column temperature was 25 ℃. RESULTS The linear range of trahydorpalmatine was 4.8-48.0 μg·mL^-1. The average recoveries was 99.82% with RSD of 1.00% (n=6). CONCLUSION The method established is sensitive, accurate and can be used for quality control of Xuedan Weichang pill.