Determination of Irbesartan in Human Plasma by HPLC

OBJECTIVE To develop a high performance liquid chromatography method for the determination of irbesartan in human plasma. METHODS The plasma were extracted by diethyl ether. The analytical column was packed with ZORBAX Extend-C₁₈. The mobile phase were acetonitrile-water-0.1% trifluoroacetic acid-0.5 mmol·L^-1 SDS and the flow rate was 0.8 mL·min^-1. The UV detection wavelength was 242 nm(0-6.5min) and 266 nm(6.5-7.8min). RESULTS Excellent liner relationship was obtained in the range of 0.05-6.00 mg·L^-1(r=0.999 9). The limit determination of irbesartan was 0.05 mg·L^-1. The relative recoveries were (103.93±1.09)%, (99.85±0.65)% and(100.95±1.21)% respectively at three concentrations, the intra-day RSD were 3.04%, 1.15% and 1.19% and inter-day RSD were 4.50%, 3.32% and 1.18%, respectively. CONCLUSION The method was accurate, simple, rapid and could be used to determine the irbesartan concentration in human plasma and for study of its pharmacokinetics.