Preparation and Evaluation of Ion-activated In Situ Gels for Matrine

OBJECTIVE To prepare the in situ gelling of matrine for ocular use, methods of quality monitoring and evaluating the preparation were established. METHODS HPLC was adopted to measure the contents of Gelrite alginate-based in situ gellings. As a complement, the results of stability studies, in vitro studies and the ability to inhibit ocular inflammation were obtained. RESULTS The pH values of formulations were 7.5-8.0, linear relationships for matrine between 10-60 μg·mL^-1 were good(r=0.999 3). The average recovery rate was 98.7%, RSD was 1.3%. The gels could prolong the contact time upon the cornea and markedly inhibit ophthalmic inflammation caused by the tincture of chili. CONCLUSION The preparation was stable, safe, anti-irritation and effective for the experimental ophthalmia, while using HPLC to control its quality was feasible.