Study on the Jaundice-Relieving and Enzyme-Reducing Effect and Its Mechanism of Total Paeony Glycoside

OBJECTIVE To research the jaundice-relieving and enzyme-reducing effect and its mechanism of total paeony glycoside (TPG). METHODS All ICR mice were randomly divided into normal group, model group, low dose group of TPG (LG), middle dose group of TPG (MG), high dose group of TPG(HG), and phenobarbital sodium group. Intrahepatic cholestatic model of mice was established after ig alpha-naphthylisothiocyanate (ANIT). Effect of drugs on TBIL, DBIL, TBA, ALT, AST, and ALP in serum, capacity of bile secretion, and uridine diphosphoglucuronyl transferase (UDPG-T) activity of liver tissue were observed on the sixth and twelfth day, respectively. Sleeping time of pentobarbitol sodium were observed on the sixth day. RESULTS Compared with the model group, all serum indices of three TPG groups degraded significantly (P<0.01 or P<0.05), except ALT of MG on the sixth day and AST of LG on the twelfth day. Capacity of bile secretion of MG and HG increased significantly (P<0.01 or P<0.05), sleeping time of pentobarbitol sodium of all three TPG groups shortened obviously (P<0.01), and UDPG-T activity of liver tissue of TPG enhanced significantly (P<0.05). CONCLUSION TPG possesses the jaundice-relieving and enzyme-reducing effect and its mechanism might be related with its effect of increasing capacity of bile secretion, enhancing the activiy of liver drug enzyme and UDPG-T of model mice.