HPLC Determination of Contents and Related Substances of Synthetic Thymosin α1

OBJECTIVE To assay the content and related substances of thymosin α₁. METHODS　HPLC was exploited to determinate the content and related substances of thymosin a1 at 215 nm. The separation was performed on Vydac C18 column (250 mm×4.6mm，5 mm) with the mobile phase of 0.02 mol·L^-1 phosphate buffer (pH 7.00)-isopropanol(95∶5) under 25 ℃. RESULTS The linearity was obtained over the range of 0.01-0.83 mg·mL^-1(r=0.999 8). The LOD was 10 ng. Related substances were 2.5%-2.6%. CONCLUTION　The method is simple, convenient, accurate and can be employed to assay the content and related substances of thymosin α₁ for its quality control.