Determination of Four Components of Compound Tetracycline Tablets by HPLC

OBJECTIVE To establish an HPLC method for the content determination of tetracycline hydrochloride, trimethoprim, vitamin B1 and vitamin B6 in compound tetracycline tablets. METHODS The Phenomenex Cl8(150 mm×4.6mm, 5 μm) column was used, the mobile phase was methanol-0.05% sodium heptanesulfonate-triethylamine(pH=3.5 adjusted by acetic acid ) (20∶80∶0.3), the flow rate was 1 mL·min^-1 and the detection wavelength was set at 246 nm. RESULTS The calibration curve linear of tetracycline hydrochloride, trimethoprim, vitamin B1 and vitamin B6 were 20-160 μg·mL^-1(r=0.999 9), 8-64 μg·mL^-1(r=0.999 9), 1-8 μg·mL^-1(r=0.999 9), 1-8 μg·mL^-1(r=0.999 9) respectively. The average recover were 100.1%, 100.3%, 99.3%, 100.5%, respectively. CONCLUSION The method is simple, accurate, reproducible, and it can be used for the quality control of compound tetracycline tablets.