Study on Nonaqueous Reversed Phase HPLC Fingerprint of Coicis Oil

OBJECTIVE　 To establish an HPLC fingerprint of coicis oil. METHODS 　High performance liquid chromatography was used for identification. Separations were performed on a Zorbax Extend C18 column (250 mm×4.6 mm, 5 µm)with an isocratic mobile phase of acetonitrile-dichloromethane (65∶35); flow rate: 0.5 mL·min^-1; analytes were detected by evaporative light-scattering detector(ELSD). RESULTS　The method of HPLC fingerprint analysis was validated and in keeping with the requirement. Seven common peaks were found in fingerprint. CONCLUSION　 All results above exhibit that the method is practicable, reproducible, and reliable as a method for quality control of coicis oil.