Determination of Cefixime by RP-HPLC

OBJECTIVE To establish a method for the determination of cefixime by RP-HPLC. METHODS Cefixime was separated on a Hypersil BDS C18 column (4.6 mm×250 mm, 10 μm) with a mixture of 0.1 mol·L-1 ammonium acetate (adjusted with ammonia solution to pH 7.0) and acetonitrile (96∶4) as mobile phase. The flow rate was 1.0 mL·min-1. The detection wavelength was 254 nm. The column temperature was 35 ℃. RESULTS The calibration curve was linear within the range of 102.38-1 433.45 μg·mL-1 for cefixime (r=0.999 9). The limit of detection and limit of quantitation were 0.18 ng and 0.60 ng respectively . Statistical analysis by t-test showed no significant differences between the results obtained by the method and by BP 2008. CONCLUSION This method is simple and selective for the determination of cefixime.