HPLC Determination of Cucurbitacin B in Radix Trichosanthis

OBJECTIVE To establish a method for the determination of cucurbitacin B in Radix trichosanthis by HPLC. METHODS The HPCL system consisted of Hypersil C18 column (4.6 mm ×250 mm, 5 μm), CH3OH-H2O(70∶30) mixture as a mobile phase, detection wavelength at 227 nm, 1.0 mL·min-1 of flow rate and column temperature at 30℃. RESULTS The linear range of cucurbitacin B was 0.136-0.816 μg·mL-1(r=0.999 7), the recovery was 99.8%, RSD=1.18%. CONCLUSION The method is simple, accurate, sensitive and reproducible. This method can be used for the determination of cucurbitacin B in Radix trichosanthis.