Study on Defatting Technology of Fructus Arctii

OBJECTIVE To study the optimum parameters of defatting technology of Fructus Arctii by the supercritical CO2 fluid and chemical constituents. METHODS Supercritical CO2 fluid extraction condition was selected by uniform design. The extraction pressure, extraction temperature, extraction time were three factors in the experiment. The oil was analyzed by GC-MS. RESULTS The optimum conditions were obtained: the extraction pressure was 32 Mpa, the extraction temperature was 40 ℃, and the extraction time was 40 minutes. In the pilot test the overall yield was 6.44% and the extracting rate of Arctigenin was 0.21%. 32 chemical constituents were separated from the oil and the major component is octadecadienoic acid. CONCLUSION Uniform design can optimize the CO2 supercritical fluid extraction process quickly and accurately with satisfactory results.