Determination of Tioguanine and Its Related Substances by RP-HPLC

OBJECTIVE To establish a reversed-phase high performance liquid chromatography (RP-HPLC) method for the determination of the tioguanine tablet and its related substances. METHODS The analytical column was Altima C18 and the mobile phase was 0.05 mol·L-1 sodium dihydrogen phosphate acid (pH 3.0). The column temperature was maintained at 35 ℃. The detection wavelength was set at 248 nm. The flow rate was 1.0 mL·min-1. RESULTS Tioguanine was linear in the range from 8 to 120 mg·L-1 (r=0.999 9). The average recoveries were 99.27%. Guanine was linear in the range of 0.1-40 mg·L-1(r=0.999 9). The limit of detection was 0.08 mg·L-1. The average recoveries were 100.23%. CONCLUSION The method was proved to be accurate, sensitive and precise and showed highly specific. It can be applied for the content assay, as well as the related substances of tioguanine tablet.