Study on HPLC Fingerprint of Ethyl-acetate Part of Radix Lindera Reflexae in Henan Province

OBJECTIVE To establish HPLC fingerprint of ethyl acetate part of Radix linderae reflexae. METHODS HPLC method was used. Chromatographic column was a Hypersil ODS2 C18(250 mm ×4.6 mm，5 μm); mobile phase was water-methanol with gradient elution；detected wavelength was 297 nm; column temperature was 30 ℃; flow rate was 1.0 mL·min-1. RESULTS The HPLC fingerprint of ethyl acetate part of Radix linderae reflexae was established based on the determination of 23 batches of Radix linderae reflexae, and the similarity was analysized. The similarity evaluation is above 0.85 except the sample S13 collected from Xixia country in Henan province. CONCLUSION The method is simple and reliable, and can be used for control the quality of Radix linderae reflexae.