Comparision of the Content Determination of Midecamycin and Acetyl Midecamycin by Turbidimetric Method

OBJECTIVE To establish a turbidimetric method for the content determination of midecamycin and acetyl midecamycin. METHODS The test organism was Staphylococcus aureus and cultured with fresh medium (pH 7.0). Adjust spore suspension of Staphylococcus aureus inoculate with a 1.2%-2.5% ratio, at 37℃±0.5℃ for about 3-4 h. RESULTS The liner range of midecamycin was 1.0-3.0 IU·mL-1, and it of acetyl midecamycin was 0.3-3.0 IU·mL-1. CONCLUSION The method is sensitive, rapid and feasible to determine midecamycin and acetyl midecamycin.