Study on the HPLC Fingerprint of Radix Scutellariae and Its Processed Pieces

OBJECTIVE To study the HPLC fingerprints of Radix Scutellariae and its processed pieces. METHODS Selecting HPLC-UV fingerprints. Using a Hypersil C18 column (200 mm×5.0 mm, 5 μm). The mobile phase was linear gradient elute with methanol-0.4% phosphoric acid-acetonitrile. Flow rate was 1.0 mL·min-1. Column temperature was 28 ℃. Detection wavelength was set at 277 nm. RESULTS There was no evident difference among the fingerprints of 10 batches of Radix Scutellariae that were normatively manufactured and processed,and the similarity of 14 batches of Radix Scutellariae was very well. CONCLUSION This method recurs well, and can be used to provide scientific basis for Radix Scutellariae and its processed pieces quanlity control mode.