Determination of Diosgenin in Gugefengtong Capsule by RP-HPLC

OBJECTIVE To establish an HPLC method for the determination of diosgenin in Gugefengtong capsule. METHODS diosgenin was separated on Hypersil ODS2 (4.6 mm 250 mm, 5 μm) column and detected at 203 nm, with methanol-water (93∶7) as mobile phase. RESULTS There was good linearity from 0.772 8 to 3.864 0 μg (Y= 443 254X＋18 856, r=0.999 1) in separation of diosgenin. The average recovery was 97.87% with RSD of 1.71%. CONCLUSION This method provides the reference to establish quality standard for Gugefengtong capsule.