Determination of Rosmarinic Acid in Spica Prunellae by HPCE

OBJECTIVE To establish an HPCE method for content determination of rosmarinic acid in Spica Prunellae. METHODS The separation was carried out in an uncoated fused silica capillary (75 μm ×50 cm). The running voltage was 18 kV. The buffer was 20 mmol·L-1 boric acid (pH 9.88)，and the wavelength was 330 nm. RESULTS The linear range of the calibration curve was 20.4-163.2 μg·mL-1(ｒ=0.999 4), and the average recovery was 97.7%，RSD was 1.6 %. CONCLUSION This method is proved to be simple, rapid, accurate and exclusive, and can be used as a new method for the determination of rosmarinic acid in Spica Prunellae.