Determination of Scutellarin Brevisapin Sustained-Release Capsules by HPLC

OBJECTIVE To develop an HPLC method of scutellarin in brevisapin sustained-release capsules. METHODS C18(4.6 mm ×150 mm, 5 μm) column with temperature of 30 ℃ was used. The mobile phase was the mixed solution of methanol- tetrahydrofuran-0.1% phosphoric acid (14∶14∶72) with the flow rate of 1.0 mL·min-1. Determination wavelength was 335 nm and the sample injected volume was 10 μL. RESULTS A good linearity of scutellarin was in the range of 24.45-293.46 μg·mL-1. The average recovery of scutellarin was 101.5% with RSD=3.616%. CONCLUSION The method is simple, accurate, and can be used for the quality control of brevisapin sustained-release capsules.