RAPD Analysis on Cynanchum Otophyllum and Its Closely Related Species

OBJECTIVE There are confused species on Cynanchum otophyllum Schneid. And distinct intraspecies variation，interspecific indentification has been done among C. otophyllum，C．Wilfordii (Maxim.) Hemsl. And C. wallichii Wight；Genetic diversity of 4 populations of C. otophyllum have been analyzed. METHODS Random amplified polymorphic DNA(RAPD) technique has been used to analyze. Genetic diversity of C. otophyllum has been evaluated by using Nei's gene diversity and Shannon's information index. The dendrogram has been constructed by UPGMA. RESULTS Ten RAPD primers have been selected to apply for random amplification. A total of 72 DNA bands are amplified among which 70(97.22%) are polymorphic. Shannon's information index is 68.52% within the populations of C. otophyllum, more than 31.48% among 4 populations of C.otophyllum; the index Gst is 30.56% among 4 populations of C. otophyllum, less than 68.44% within the populations of C.otophyllum. The dendrogram of genetic relationship among three species has been constructed by using UPGMA. CONCLUSION RAPD is amplified to distinguish C. wallichii form C. otophyllum and C．Wilfordii. Although there is distinct differentiation among populations of C. otophyllum, the great genetic variation happened within populations.