Effect of HPLC Fingerprint of Radix Bupleuri with Different Fertilizers at Different Growth Stages

OBJECTIVE To establish a method of fingerprint analysis on Radix Bupleuri by HPLC-PDA. METHODS The HPLC method was used with a SunFireTM C18 column (250 m×4.6 mm,5 μm).The mobile phase was acetonitrile-0.1% phosphricacid and the detective wavelength was set at 210 nm with the column temperature of 30℃. RESULTS Establishing the fingerprint of Radix Bupleuri,15 common peaks were found in the HPLC fingerprint of Radix Bupleuri and good similarities with correlation coefficients higher than 0.90 were found in fingerprints between the herbs and standard fin/gerprint, which can be utilized for the identification of Radix Bupleuri. CONCLUSION The method is accuate, simple, and could be used for quality control of Radix Bupleuri.