Determination of Doxorubicin Liposomes Modified with Transferrin in PCR Mice by HPLC With Fluorescence Detector

OBJECTIVE To establish a sensitive and specific method for the determination of doxorubicin liposomes modified with transferrin in PCR mice by HPLC with fluorescence detector. METHODS RP-HPLC with fluorescence detector was used. ADIKMA Diamonsil C18 column (4.6 mm×150 mm，5 μm) was used with the mobile phase of acetonitrile-5 mmol·L-1 ammonium acetate (30∶70). The detection wavelength was EX 480 nm，EM 550 nm and the temperature was at 30 ℃. The flow velocity was 1.0 mL·min-1. RESULTS The calibration curve showed a good linearity. The result of the extraction recovery, the intra-day and inter-day RSD of doxorubicin were all analytical for the determination. CONCLUSION The method is sensitive, fast, precise and reliable to operate, and is suitable for the analysis of doxorubicin liposomes modified with transferrin in PCR mice.