Determination of Oleanolic Acid in Weilingxian by HPLC-ELSD

OBJECTIVE To establish an HPLC-ELSD method for the determination of oleanolic acid in Weilingxian. METHODS Using SumFireTM C18 column(4.6 mm×250 mm，5 μm) at 40 ℃，acetonitrile -water(85∶15) was used as the moble phase，the flow rate was 1.0 mL·min-1. An evaporative light-scattering detector(ELSD) was used as detector with gas flow rate of 2.3 L·min-1 and drife tube temperature of 85 ℃. RESULTS The oleanolic acid had a good linearity relationship between 0.214-1.92 μg(r=0.999 9). The average recovery was 98.8% with RSD = 0.9%(n=6). CONCLUSION The method is simple，rapid and accurate，suitable for the quality control of Weilingxian.