Determination of Four Compounds in Fat-soluble Vitamin for Injection by HPLC

OBJECTIVE To establish an HPLC method for simultaneous determination of retinol palmitate，vitamin D2，vitamin E and vitamin K1 in fat-soluble vitamin for injection. METHODS An Intersil (ODS-2) C18 (150 mm×4.6 mm，5 μm) was used as separation column. Methanol- acetonitrile- ethanol-water (17∶58∶25∶5) was used as the mobile phase. Flow rate was 1.5 mL·min -1. The detection wavelength was set at 265 nm. RESULTS The linear ranges for retinol palmitate，vitamin D2, vitamin E and vitamin K1 were 1.19-2.78 μg，6.14-14.33 ng，10.92-25.48 μg，0.19-0.44 μg, and the average recoveries were 99.70% (RSD=0.48%), 100.13% (RSD=0.88%)，99.74% (RSD=0.69%), 99.97% (RSD=0.59%), respectively. CONCLUSION The method is simple，accurate，reliable. It can be used for the quality control of fat-soluble vitamin for injection.