Studies on the Chemical Constituents in Ranunculus muricatus L.

OBJECTIVE To isolate and determine the chemical constituents from Shengzhou Ranunculus muricatu L. . METHODS Eight compounds were isolated and purified by extraction and different kinds of column chromatography. The structures were determined on the basis of extensive spectral analysis such as EI-MS， 1H-NMR，13C-NMR and DEPT. RESULTS The structures were elucidated as stigmasta-4-ene-3，6-dione(I)，stigmasterol(Ⅱ)，anemonin (Ⅲ)，scoparone (IV)，β-sitostero1(V), protocatechuic aldehyde (Ⅵ)，protocatechuic acid(Ⅶ) and 1uteolin (Ⅷ), respectively. CONCLUSION Compounds( I，Ⅳ，V，Ⅵ) were isolated from Ranunculus muricatus for the first time.