Determination of Gatifloxacin and Moxifloxacin in Whole Blood by HPLC Coupled with Fluorescence Detection

OBJECTIVE To develop a sensitive and accurate method for the determination of gatifloxacin and moxifloxacin in whole blood by high-performance liquid chromatography coupled with fluorescence detection(HPLC-FLD). METHODS After gatifloxacin and moxifloxacin in whole blood were extracted by phosphate buffer, the sample was cleaned with solid-phase extraction (SPE) using Oasis MAX cartridges.The separation was performed on Cloversil - C18 column（3.0 mm ×150 mm, 5 μm）using the mobile phase consisting of methanol-acetonitrile-0.2% formic acid(15∶15∶70).Detection was carried out on a fluorescence detection at λex 288 nm and λem 493 nm. RESULTS Calibration curves were linear within the range of 15.0-240.0 μg·L-1, and the extraction recoveries were from 97.7% to 101.3%, the RSDs were less than 6.0%.The limits of quantification were found to be 15.0 μg·L-1.CONCLUSION This method is found to be simple,sensitive,little interferential and good specificity for the determination of gatifloxacin and moxifloxacin in whole blood.