Neuroprotective Effect of Cerebrospinal Fluid of Nimodipine on Various PC12 Cell Injuries in Vitro

OBJECTIVE To explore the standard conditions and application of the cerebrospinal fluid （CSF）pharmacological method, by investigating the neuroprotective effect of CSF with nimodipine on PC12 cells injured by various factors. METHODS The effect of rat CSF with nimodipine on injured PC12 cells induced by potassium chloride (KCl) was observed by 3-(4,5-dimethyl- 2-thiazol-2, 5-diphenyl-2H-tetrazolium bromide (MTT assay under various experimental conditions (dosage regiment, addition level and treatment process, then its neuroprotective effect under certain conditions on PC12 cells injured by hydrogen peroxide (H2O2, sodium dithionite (Na2S2O4 and L-glutamate sodium were determined as well. RESULTS The viability of PC12 cells injured by KCl increased with the addition level of CSF with nimodipine, and at the same addition level, CSF of rat administrating nimodipine repetitiously (twice·d-1×3.5d) exerted higher protective efficacy on PC12 cells than that of single administration.The raw CSF with nimodipine and the CSF with nimodipine treated by heat (56 ℃), ethanol or acetone had notable protective effects on injured PC12 cells (P<0.01, and there was no obvious statistical difference among them. The similar distinct protective effects of CSF with nimodipine under certain conditions on PC12 cells injured by H2O2, Na2S2O4 and L-glutamate sodium were also observed. CONCLUSION It is proposed that the CSF with nimodipine having significant protective effects on PC12 cells injuries caused by multi-factors such as KCl, Na2S2O4 and H2O2 etc may be prepared by 10 rat CSF without treating after administrating drugs repetitiously.