Determination of Liquiritigenin and Glycyrrhizic Acid in Xuanmaiganjie Granule by HPLC

OBJECTIVE To establish an HPLC method for the determination of liquiritigenin and glycyrrhizic acid in Xuanmaiganjie granule.METHODS A LiChrospher C18column (250 mm×4.6 mm,5 μm) was used，the mobile phase was acetonitrile-water（1.2% methanoic acid） for gradient elution. The detection wavelength was set at 254 nm.RESULTS The linear range was 0.005-0.5 mg·mL-1.The regression equation of liquiritigenin was Y=5 735.789 7X-2.702 7(r=0.999 9)，and the average recovery was 99.7%（RSD=1.72%，n=9）.The regression equation of glycyrrhizic acid was Y=6 968.182 6X-3.448 4（r=0.999 8），and the average recovery was 100.8%（RSD=1.31%，n=9）.CONCLUSION The method is convenient and accurate. It can be used to determine the contents of liquiritigenin and glycyrrhizic acid in Xuanmaiganjie granule.