LC-MS Detection of Prednisone Acetate of Compound Chuanlinng Dingchuan Capsules

OBJECTIVE To develop a specific method for the determination of prednisone acetate illegally mixed into drug. The liquid chromatography-mass spectrometry method was used. The Venusil MP C18 column was used with amobile phase was 0.01 mol·L-1 ammonium acetate solution（with 0.1%）acetonitrile （60∶40）. Prednisone acetate illegally mixed into drug was identified according to the chromatographic behavior and mass spectral data by comparison with reference substances.RESULTS Prednisone acetate was found in drug. The average recovery was 98.62% (n=6). CONCLUSION The method is selective, sensitive and can be used to detect prednisone acetate illegally mixed into drug.