Determination of Cinobufagin and Recibufogenin in Tongqiao Yixin Pill by HPLC

OBJECTIVE To establish an HPLC method for the determination of cinobufagin and recibufogenin in Tongqiao Yixin pill. METHODS The analysis was performed on a SinoChrom ODS2 column. The mobile phase consisted of 0.5% potassium phosphate（pH 3.2）acetonitrile (52∶48), and the flow rate was 1.0 mL·min-1. The detection wavelength was 296 nm. RESULTS The average recovery and RSD were 99.77% and 0.49% for cinobufagin, 99.39% and 0.60% for recibufogenin, respectively. CONCLUSION This method is simple and reliable for the quality control of Tongqiao Yixin pills.