Simultaneous Determination of Silymarin and Schizandrol in Compound Yi Ganling Tablets by HPLC

OBJECTIVE To simultaneous determine the content of silymarin and schizandrol in compound Yi Ganling tablets by HPLC. METHODS The analytical column was a Diamonsil C18 column (250 mm×4.6 mm,5 μm). The mobile phase consisted of methanol water. The gradient condition was 0→60 min, methanol 40%→75% and water 60%→25%. The detection wavelengths of silymarin and schizandrol were at 287 nm and 250 nm. RESULTS The calibration curves of silymarin and schizandrol were in good linearity over the ranges of 4.96-39.68 μg·mL-1(r=0.999 8) and 4.00-32.00 μg·mL-1(r=0.999 8), the average recoveries were 98.8% and 99.5%，RSD were 0.96% and 0.59%. CONCLUSION The method is simple, rapid, reliable and can be used for the drug control.