Simultaneous Determination of Salvianolic Acid B, Icariin in Bindouling Oral Liquid by HPLC

To establish a method for simultaneously determining effective components in Bindouling oral liquid by RP-HPLC. METHODS HPLC analysis was performed on Alltech C18 column (250 mm×4.6 mm,5 μm) with a mixture of acetonitrile (A) and 0.2% acetic acid-water(B) as mobile phase in gradient mode.The column temperature was set at 30 ℃ and the flow rate was 1.0 mL·min-1 with the wavelength at 270 nm.RESULTS The two effective components could be detected by this method, and their contents could be determined. A good linearity of salvianolic acid B,icariin was in the range of 25.81-413.0 μg·mL-1,2.97-59.36 μg·mL-1，respectively.The average recoveries were 99.77% and 100.1% with RSD of 0.5% and 2.3%,respectively. CONCLUSION The method is simple, feasible and reliable.It can be used for the quality control of Bindouling oral liquid.