Determination of the Content of Saikosaponin a in Different Chailing Decoction Granules by HPLC

OBJECTIVE To establish a simple method and compare for the determination of the content of Saikosaponin a in different Chailing decoction granules by HPLC. METHODS Kromasil C18 chromatographic column（250 mm×4.6 mm，5 μm）was used.The mobile phase was methanol-water(75∶25).The flow rate was 0.8 mL·min-1, the column temperature was 30 ℃, and the detection wavelength was set at 210 nm.RESULTS The linear relation range of Saikosaporin a was 4.0-15.0 μg( r=0.999 9).The average recovery was 100.39％ with the RSD of 0.80%. CONCLUSION Japanese Jincuntang were better than Imitating Japanese method Chailing decoction granules which were better than Chinese ordinary method granule on the content of Saikosaponin a. The method is simple, sensitive, and feasible for the determination of Saikosaponin a and quality control of Chailing decoction granule.