The Quality Analysis of Defferent Areas of Henan′s Spica Prunellae

OBJECTIVE To establish an HPLC method for the determination of ursolic acid and oleanolic acid in different area of Henan′s Spica Prunellae. METHODS The ursolic acid and oleanolic acid separation was performed on a column: Betasil C18(250 mm×4.6 mm,5 μm) column, mobile phase was natrium dihydrogen phosphate solution (7.8 g→1000 mL)-methanol (14∶86), the flow rate was 0.8 mL·min-1, the column temperature was 25 ℃, the detective wavelength was 210 nm. RESULTS The ursolic acid calibration curve showed good linearity in the range of 0.554-5.34 μg(r=0.999 7), the average recovery was 97.45%(RSD=0.92%). The oleanolic acid calibration curve showed good linearity in the range of 0.28-2.24 μg (r=0.999 4), the average recovery was 97.61%(RSD=0.87%). CONCLUSION The method is simple,accurate and sensitive.