Determination of Mannitol and Glucose in the Compound Mannitol Injection by HPLC-RI

OBJECTIVE To establish an HPLC-RI method for the determination of the contents of mannitol and glucose in Compound Mannitol Injection.METHODSThe HPLC-RI system consisted of strong acid cation exchange resin (Ca2+)( Sugar Pak Ⅰ300 mm×6.5 mm) as stationary phase, water as mobile phase at the flow rate of 0.5 mL ·min-1.The column temperature was(85±1)℃.The differential refractive index detector temperature was 40 ℃. RESULTSThe linear of glucose was 0.05-5 mg·mL-1,Y=640 151X-3 719.6(r=1.000 0,n=7),the average recovery was 100.1%( RSD=0.89%,n=9).The linear of mannitol was 0.15-15 mg·mL-1,Y= 643 467X-15 373 (r=0.999 8,n=7),the average recovery was 100.1% (RSD=0.73%,n=9).CONCLUSIONThis method is simple,accurate and better reproducibility,can meet with the requirement of the determination of the contents of mannitol and glucose.