Simultaneous Determination of Alkaloids by HPLC in Rat Plasma after Kexieling Tablets Administration

OBJECTIVE To establish an HPLC method for simultaneously determining the alkaloids of Kexieling tablets in rat plasma. METHODS The plasma samples were determined at 220 nm wavelength by reversed-phase HPLC and ultraviolet detection following oral administration of Kexieling tablets. RESULTS Sophoridine and matrine with 350 and 100 ng·mL-1of limits of quantitation in plasma, respectively, can be accurately quantitated, and the recovery of both analytes was greater than 98%.CONCLUSION The method is simple,accurate and can be referenced for pharmacokinetic study of Kexieling tablets.