Detection of Bacterial Endotoxin in Iohexol by the Method of Kinetic Turbidimetric Limulus Test

OBJECTIVE To detect the quantity of endotoxin in iohexol for injection by the method of the kinetic turbidmetric limulus test. METHODS The kinetic turbidmetric limulus test was applied to detect the quantity of endotoxin in several samples. The standard endotoxin working curve was set up and the interference test was studied to range the effective concentrations of sample.RESULTS The diluted concentration of sample preparation down to 10 mg·mL-1 was effective to eliminate the interference. The endotoxin recovery was more than 50% and less than 200%. The endotoxin level in samples could be quantified exactly, and the results were accorded with the results of the pyrogen test. CONCLUSION The kinetic turbidmetric limulus test can be used to quantitate the endotoxin level in iohexol.