Determination of Three Components in Compound Aspirin Tablets by HPLC

OBJECTIVE To determine acetyl salicylic acid,caffeine and phenacetin in compound aspirin tablets. METHODS The chromatographic conditions included Symmetry C18column（4.6 mm×150 mm, 5 μm）.The mobile phase was phosphate salt buffer(pH6.5)-acetonitrile(78∶22).The flow rate was 1.0 mL·min-1.The detection wavelength was 270 nm. RESULTS Acetyl salicylic acid,caffeine and phenacetin had good linear relationship in the range of 8.80-70.42 μg· mL-1（r=0.999 9），1.42-11.34μg· mL-1(r=0.999 9) and 6.12-48.92 μg· mL-1 (r=0.999 9).Their recoveries were 101.2%（RSD=0.7%，n=6），99.1%（RSD=0.8%，n=6） and 100.3%（RSD=0.7%，n=6）,respectively.CONCLUSION This method could be used for the determination of three components in compound aspirin tablets without separation.