Determination of Active Constitutents in Folium Eucommiae by RP-HPLC

OBJECTIVE To establish a new reversed phase high-performance liquid chromatography (RP-HPLC) method for the determination of chlorogenic acid and genipoide, the active constitutents in folium eucommiae simultaneously. METHODS YMC-pack ODS-A column (250 mm×4. 6 mm, 5 μm) was used and the mobile phase was methanol-water-acetic acid (20∶80∶1.5) at the flow rate of 1.0 mL·min-1. The detection wavelength was 237 nm and the temperature of column was 30 ℃. RESULTS The linear range of chlorogenic acid and genipoide were 0.041 6-0.624 0 μg(r=0.999 2) and 0.081 6-1.224 0 μg(r=0.998 9). The average recoveries were 102.92% and 102.35%,respectively. The RSD were 1.49% and 1.20%. The content of chlorogenic acid and genipoide in folium eucommiae were 2.801 mg·g-1 and 0.273 mg·g-1. CONCLUSION The method is simple, accurate and reliability, with good repeatability, and can be used for the quality control of folium eucommiae.