Study on the Fermentation Condition of Coenzyme Q10 byCandida Tropicalis

OBJECTIVE In order to achieve higher yield of CoQ10,Different fermentation factors influencing CoQ10 production with yeast Candida Tropicalis were studied. METHODS CoQ10 was extracted by alcohol and measured by UV spectrophotometry.RESULTS Glucose and bean peptone were favourite for cell growth and CoQ10 accumulation. Phosphorus sources of KH2PO4 and K2HPO4 were important factors influencing the cell growth and CoQ10 accumulation. Meanwhile, MnSO4 can enhance the CoQ10 accumulation in the cells. The culture conditions were primarilay optimized: initial pH 8.0, inoculum size 4.0%, volume of medium 40 mL/250 mL shake flask, 72 h, glucose 4.0%, bean peptone 5.0%. Under the condition, CoQ10 accumulation reached 70 μg·mL-1.CONCLUSION Candida Tropicalie at the Co10 straina of high yield was practicable and marketable.