Optimizing Preparation of Encapsulated Octafluoropropane Albumin Microspheres by Orthogonal Design

OBJECTIVE To optimize preparation of encapsulated octafluoropropane albumin microspheres.METHODS The microspheres in suspension were produced by sonication of 20 mL of 1 % human serum albumin solution in the presence of octafluoroprane gas. Orthogonal design was used for studying the octafluoropropane gas flow rate, sonication intensity and process temperature of termination on the diameter and concentration of microspheres.RESULTS The best optimization process was obtained as follows: 90 % intensity as the sonication power, 2 mL per 5 s as the gas flow rate,77 ℃ as the terminal process temperature.CONCLUSION The microspheres prepared by this method are stable.