Purification and Determination of Polysaccharide from Scutellaria barbata D.Don and Tumor-Inhibitory Effect of Polysaccharide in Mice Bearing of Sarcoma-180

OBJECTIVE To detect the content of purificatus polysaccharide from SPS, and compare the tumor-inhibitory of crude materials and purificated polysaccharide and provide some references for research on chemistry and pharmacology of polysaccharide from cutellaria barbata D.Don.METHODS UV-spectrophotometer and phenol-sulfuric method were used, the detection wavelength was 490 nm and tumor-inhibitory effect of polysaccharide was detected through intracorporeal test. RESULTS The percent of purificatus polysaccharide was 54.12% ,average recovery was 99.66%, the rate of inhibiting tumor of crude and purificatus polysaccharide were 45.96% and 22.14% respectively.CONCLUSION This method is simple, convenient, reliable and the anti-tumor activity of crude polysaccharide is better than that of purificatus polysaccharide.