HPLC Method for Determination of Phenytoin and its Metabolite 4'-Hydroxyphenytoin in Human Urine
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Graphical Abstract
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Abstract
A RH-HPLC procedure had been developed for the determination of phenytoin(PHT)and its main metabolite 4'-hydroxyphenytoin(HPPH)in human urine.PHT and HPPH were extr-acted from urine using ether. HPLC was performed on a Bio-Sil(?) ODS column,with a mobilep
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